全文获取类型
收费全文 | 171篇 |
免费 | 14篇 |
专业分类
化学 | 169篇 |
晶体学 | 1篇 |
数学 | 1篇 |
物理学 | 14篇 |
出版年
2021年 | 4篇 |
2020年 | 6篇 |
2019年 | 8篇 |
2018年 | 1篇 |
2016年 | 7篇 |
2015年 | 4篇 |
2014年 | 5篇 |
2013年 | 13篇 |
2012年 | 17篇 |
2011年 | 16篇 |
2010年 | 3篇 |
2009年 | 5篇 |
2008年 | 5篇 |
2007年 | 9篇 |
2006年 | 13篇 |
2005年 | 14篇 |
2004年 | 8篇 |
2003年 | 11篇 |
2002年 | 7篇 |
2001年 | 7篇 |
2000年 | 4篇 |
1999年 | 6篇 |
1998年 | 1篇 |
1996年 | 1篇 |
1992年 | 1篇 |
1991年 | 2篇 |
1989年 | 3篇 |
1986年 | 1篇 |
1985年 | 2篇 |
1982年 | 1篇 |
排序方式: 共有185条查询结果,搜索用时 31 毫秒
91.
Yoshida N Abe Y Shigeta H Nakajima A Ohsaki H Hashimoto K Watanabe T 《Journal of the American Chemical Society》2006,128(3):743-747
Flat films of methyl methacrylate-fluoroalkyl methacrylate copolymers were prepared, and their hydrophobicity was investigated. It was revealed that the F concentration directly affects the static hydrophobicity on the flat polymer surface in a systematic manner. Furthermore, the sliding behavior of a water droplet on these surfaces depends on the static hydrophobicity; the sliding motion changes from constant velocity to constant acceleration with an increase in the water contact angle. 相似文献
92.
Terai T Kikuchi K Iwasawa SY Kawabe T Hirata Y Urano Y Nagano T 《Journal of the American Chemical Society》2006,128(21):6938-6946
Luminescent lanthanide complexes (Tb(3+), Eu(3+), etc.) have excellent properties for biological applications, including extraordinarily long lifetimes and large Stokes shifts. However, there have been few reports of lanthanide-based functional probes, because of the difficulty in designing suitable complexes with a luminescent on/off switch. Here, we have synthesized a series of complexes which consist of three moieties: a lanthanide chelate, an antenna, and a luminescence off/on switch. The antenna is an aromatic ring which absorbs light and transmits its energy to the metal, and the switch is a benzene derivative with a different HOMO level. If the HOMO level is higher than a certain threshold, the complex emits no luminescence at all, which indicates that the lanthanide luminescence can be modulated by photoinduced electron transfer (PeT) from the switch to the sensitizer. This approach to control lanthanide luminescence makes possible the rational design of functional lanthanide complexes, in which the luminescence property is altered by a biological reaction. To exemplify the utility of our approach to the design of lanthanide complexes with a switch, we have developed a novel protease probe, which undergoes a significant change in luminescence intensity upon enzymatic cleavage of the substrate peptide. This probe, combined with time-resolved measurements, was confirmed in model experiments to be useful for the screening of inhibitors, as well as for clinical diagnosis. 相似文献
93.
Design and synthesis of an enzyme activity-based labeling molecule with fluorescence spectral change
Komatsu T Kikuchi K Takakusa H Hanaoka K Ueno T Kamiya M Urano Y Nagano T 《Journal of the American Chemical Society》2006,128(50):15946-15947
Methods of covalent labeling of a specific tag protein with small-molecular dyes play an important role in studying dynamic behaviors of proteins in living cells. On the basis of quinone methide chemistry, we designed and synthesized a beta-galactosidase labeling probe, CMFbeta-gal, which shows a fluorescence wavelength change accompanying the labeling reaction, owing to fluorescence resonance energy transfer (FRET). Since the FRET efficiency changes accompanying the labeling reaction, fluorescence of labeled protein can be observed separately from that of the unreacted probe, so immediate detection of the target protein is possible. This is the first report of a protein labeling probe which features a change of fluorescence wavelength upon reaction, allowing the labeled protein to be detected even in the presence of unreacted probe. 相似文献
94.
95.
Dr. Koji Numasawa Prof. Kenjiro Hanaoka Naoko Saito Prof. Yoshifumi Yamaguchi Takayuki Ikeno Honami Echizen Dr. Masahiro Yasunaga Dr. Toru Komatsu Dr. Tasuku Ueno Prof. Masayuki Miura Prof. Tetsuo Nagano Prof. Yasuteru Urano 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2020,132(15):6071-6076
Folate receptors (FRs) are membrane proteins involved in folic acid uptake, and the alpha isoform (FR-α) is overexpressed in ovarian and endometrial cancer cells. For fluorescence imaging of FRs in vivo, the near-infrared (NIR) region (650–900 nm), in which tissue penetration is high and autofluorescence is low, is optimal, but existing NIR fluorescent probes targeting FR-α show high non-specific tissue adsorption, and require prolonged washout to visualize tumors. We have designed and synthesized a new NIR fluorescent probe, FolateSiR-1 , utilizing a Si-rhodamine fluorophore having a carboxy group at the benzene moiety, coupled to a folate ligand moiety through a negatively charged tripeptide linker. This probe exhibits very low background fluorescence and afforded a tumor-to-background ratio (TBR) of up to 83 in FR-expressing tumor-bearing mice within 30 min. Thus, FolateSiR-1 has the potential to contribute to the research in the field of biology and the clinical medicine. 相似文献
96.
Koji Numasawa Kenjiro Hanaoka Naoko Saito Yoshifumi Yamaguchi Takayuki Ikeno Honami Echizen Masahiro Yasunaga Toru Komatsu Tasuku Ueno Masayuki Miura Tetsuo Nagano Yasuteru Urano 《Angewandte Chemie (International ed. in English)》2020,59(15):6015-6020
Folate receptors (FRs) are membrane proteins involved in folic acid uptake, and the alpha isoform (FR‐α) is overexpressed in ovarian and endometrial cancer cells. For fluorescence imaging of FRs in vivo, the near‐infrared (NIR) region (650–900 nm), in which tissue penetration is high and autofluorescence is low, is optimal, but existing NIR fluorescent probes targeting FR‐α show high non‐specific tissue adsorption, and require prolonged washout to visualize tumors. We have designed and synthesized a new NIR fluorescent probe, FolateSiR‐1 , utilizing a Si‐rhodamine fluorophore having a carboxy group at the benzene moiety, coupled to a folate ligand moiety through a negatively charged tripeptide linker. This probe exhibits very low background fluorescence and afforded a tumor‐to‐background ratio (TBR) of up to 83 in FR‐expressing tumor‐bearing mice within 30 min. Thus, FolateSiR‐1 has the potential to contribute to the research in the field of biology and the clinical medicine. 相似文献
97.
I. Shigeta Y. Tanaka F. Ichikawa Y. Asano 《The European Physical Journal B - Condensed Matter and Complex Systems》2006,54(2):141-149
We propose a new approach of smearing origins of a zero-bias
conductance peak (ZBCP) in high-Tc superconductor tunnel
junctions through the analysis based on the circuit theory for a
d-wave pairing symmetry.
The circuit theory has been recently developed from conventional
superconductors to unconventional superconductors.
The ZBCP frequently appears in line shapes for this theory, in which the
total resistance was constructed by taking account of the effects
between a d-wave superconductor and a diffusive normal metal (DN) at a
junction interface, including the midgap Andreev resonant states (MARS),
the coherent Andreev reflection (CAR) and the proximity effect.
Therefore, we have analyzed experimental spectra with the ZBCP of
Ag-SiO-Bi2Sr2CaCu2O8+δ (Bi-2212) planar tunnel
junctions for the {110}-oriented direction by using a simplified
formula of the circuit theory for d-wave superconductors.
The fitting results reveal that the spectral features of the ZBCP are
well explained by the circuit theory not only excluding the Dynes's
broadening factor but also considering only the MARS and the DN
resistance.
Thus, the ZBCP behaviors are understood to be consistent with those of
recent studies on the circuit theory extended to the systems containing
d-wave superconductor tunnel junctions. 相似文献
98.
Naoya Yoshida Yuu Abe Hiroaki Shigeta Kazuyuki Takami Hisashi Osaki Toshiya Watanabe Kazuhito Hashimoto Akira Nakajima 《Journal of Sol-Gel Science and Technology》2004,31(1-3):195-199
Transparent hydrophobic polymer coating was prepared from methacrylate copolymer with molecular modeling in order to improve hydrophobicity and durability. Organic-inorganic hybridization improved the durability of the thin films. Furthermore the control of hydrophobicity and water droplet sliding properties of the flat coating was successfully performed. Water droplet sliding angle depended on the chemical structure of the materials, not on the hydrophobicity, while sliding motion simply depended on the hydrophobicity. 相似文献
99.
Komatsu T Oushiki D Takeda A Miyamura M Ueno T Terai T Hanaoka K Urano Y Mineno T Nagano T 《Chemical communications (Cambridge, England)》2011,47(36):10055-10057
We studied the photobleaching of a library of boron dipyrromethene (BODIPY) derivatives with a range of electron densities, and found that the photobleaching rate is influenced by the electron-withdrawing capacity of the substituents. Electron-deficient BODIPYs generated less singlet oxygen, were less reactive to singlet oxygen, and were highly resistant to photobleaching. We confirmed the utility of one of these fluorophores, 2,6-diCO(2)R-BDP, for visualizing EGF receptor dynamics in cells expressing an SNAP-tagged EGF receptor. 相似文献